This hyperbolic function results from the binding interactions of macromolecules and smaller ligand molecules and is a branch of biochemcal kinetics. This type of kinetics is often treated in advanced undergraduate biochemistry courses or in qualifying graduate courses. The goal of these kinetic studies is to determine both the binding affinity of the smaller ligand molecule for the macromolecule and the number of potential binding sites available to the ligand often using radiolabelled ligands.
This sketch explores the Scatchard plot for two binding sites.
See also:
Two hypothetical Scatchard sum: [url]http://tube.geogebra.org/material/show/id/1498145[/url]
Michaelis Menten graphs:
[url]http://tube.geogebra.org/material/show/id/1491085[/url]
